Detection Of Apoptosis Of Ht1080 Cells By Fluoresc

Detection of programmed electric carrel shoemakers last of HT1080 Cells by Fluorescence worked up Cell Scanning Introduction Apoptosis or programmed cell death is an inherently programmed phenomenan (Kerr et al, 1972) that occurs in each(prenominal) multicellular organisms, described as the opposite of mitosis, necessary in providing homeostasis and preventing uncontrollable cell proliferation that whitethorn whizz to tumour strivingation. humiliation is also a form of cell death, polar to caspase-mediated cell death in that it is externally bring forth by factors such(prenominal) as injury, infection, poisoning and inflammation. Apoptosis operates via 2 mechanisms, an intrinsic pathway initiated by the release of cytochrome c from mitochondrial membranes, or an extrinsic pathway initiated by the activation of surface death receptors by proapoptotic molecules such as caspase 8. Both pathways however lead to common morphological and biochemical features, that are char acteristic of apoptosis, such as DNA fragmentation, chromatin condensation, membrane blebbing, cell shoplifting and formation of apoptotic bodies (Kerr et al, 1972). The formation of apoptotic bodies during primordial stages of apoptosis, is ca utilise by the inversion of phosphatidylserine (PS), from the informal plasma membrane to the exterior membrane, and is necessary for phagocyte citation of the apoptotic cell.

The charge of PS, is indicative of cells in the early stage of apoptosis and can be spy using Annexin-V, a phospholipid covering protein with a high kinship toward PS in the presence of calcium i ons. Annexin-V is usually combine with fluo! rescein isothiocyanate (FITC), a light molecule that allows the detection of apoptotic cells by flow cytometry. Figure 1: Translocation of PS to the outer cell membrane, and detection by Annexin-V binding. (MCBG3004 laboratory Manual, 2011). Fluorescence activated cell see (FACS), is an analytical technique used to identify and separate different cells types by using light marker molecules such as FITC, that emit light of...If you command to get a dependable essay, order it on our website: BestEssayCheap.com

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